|
The LPO-CC assay is a direct measurement of lipid peroxides
correlating with serum lipid peroxides at 98 to 99%. Urine
samples are stable for weeks and do not require refrigeration
or freezing.
The LPO-CC assay specifically and directly measures LPO.
In the presence of hemoglobin, lipid peroxides are reduced
to hydroxyl derivatives (lipid alcohols) which oxidatively
cleave colorless MCDP into methylene blue.
Urinary Lipid Peroxides reference range: 1.0 - 7.5nM/mg
creatinine.
Ideal range: < 6.0 nM/mg creatinine.
Urinary Lipid Peroxides in the upper end of the reference
range (i.e. levels > 6.5 nM/mg creatinine and < 7.5
nM/mg creatinine) may be clinically relevant. Antioxidants
should be considered and retested after 4 weeks. Levels >
7.5 creatinine should be monitored closely. Aggressive anti-oxidative
therapy should be considered as well as thorough investigation
of cause(s) of increases in free radical activity.
References
1. Ohishi, N., Ohkawa, H., Miike, A., Tatano, T., and Yagi,
K. (1985). Biochem. Int. 10:205-211.
2. Tateishi, T., Yoshimine, N., and Kuzuva, F. (1987). Exp.
Gerantol. 22:103-111
3. Pervaiz, S., Harriman, A., Gulliya, K.S. (1992). Free Radical
Biol. Med. 12:389-396.
4. Ma, Y., Stone, W., LeClaire, J. (1994). P.S .E.B.M. 206:53-59.
5. Supnet, M., David-Cu, R., and Walther, F. (1994). Ped.
Res. 36:283-287
6. Wang, M.-Y., and Liehr, J. (1994), J. Biol. Chem. 269:284-291
|
